脱细胞化细胞外基质材料对小鼠巨噬细胞活性的影响

炬文 马(承德医学院基础医学院免疫学教研室，中国)
鸿儒 宋(河北北方学院医学检验学院免疫学教研室，中国)

摘要

目的：研究脱细胞化的细胞外基质材料对于小鼠巨噬细胞活性的影响；方法：通过流式细胞术鉴定巨噬细胞特异性表面标记物；通过Live/Dead染色方法检测脱细胞化细胞外基质材料提取物对于巨噬细胞活性的影响；通过Annexin-V和PI双染流式检测凋亡方法检测脱细胞化细胞外基质材料对于巨噬细胞凋亡的影响。结果：Live/Dead染色结果显示脱细胞化细胞外基质材料提取物培养中的巨噬细胞具有良好的活性；流式细胞术结果显示脱细胞化细胞外基质提取物培养的巨噬细胞凋亡细胞与完全培养基没有区别；结论：脱细胞化细胞外基质材料提取物对于巨噬细胞没有细胞毒性。

关键词

脱细胞化细胞外基质材料（dECM）；细胞活性；小鼠

参考

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DOI: http://dx.doi.org/10.12345/yzlcyxzz.v7i9.21200