SYT7 调控 HMGB3 在非小细胞肺癌中的作用及机制的初步研究

亚睿 张(承德医学院附属医院，中国)
根 滕(承德医学院附属医院，中国)
立广 张(承德医学院附属医院，中国)
迪 吴(承德医学院附属医院，中国)
翠敏 朱(承德医学院附属医院，中国)

摘要

目的：检测NSCLC细胞肺腺癌细胞系H1299和肺鳞癌细胞系H520中SYT7和HMGB3的表达，探究SYT7是否通过调控HMGB3影响NSCLC细胞的进展。方法：通过实时荧光定量PCR技术（qRT-PCR）和蛋白免疫印迹实验（Western blot）检测H1299和H520细胞系中SYT7和HMGB3的表达情况。在H1299和H520细胞系中分别转染siSYT7和siNC，采用qRT-PCR和Western blot检测HMGB3的表达。结果：细胞实验证明，SYT7、HMGB3在H1299和H520细胞系中mRNA和蛋白水平高表达。敲减SYT7后，HMGB3的mRNA和蛋白表达均下降。结论：该实验证明SYT7可能通过调控HMGB3促进肺癌的进展。

关键词

非小细胞肺癌；突触结合蛋白7；高迁移率组盒3

参考

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DOI: http://dx.doi.org/10.12345/yzlcyxzz.v8i1.23403