Molecular Diagnostics for Rapid Identification of Bovine Pathogens

khan Shama khan(Department of Clinical Medicine and Surgery, University of Agriculture, Faisalabad-38000, Pakistan)

Abstract

This paper provides a comprehensive review of the application of molecular diagnostic techniques for the rapid identification of bovine pathogens. Molecular diagnostics, including polymerase chain reaction (PCR), loop-mediated isothermal amplification (LAMP), and nucleic acid sequencing, have emerged as indispensable tools for the swift, sensitive, and precise detection of bovine pathogens. The article delves into the fundamental principles underlying molecular diagnostic techniques, highlights commonly targeted pathogens, explores diverse application scenarios, and outlines future directions in the field. Through this exposition, readers will gain a deeper understanding of the pivotal role played by molecular diagnostic techniques in bovine health management, as well as their potential for disease control and prevention. Furthermore, as these technologies continue to evolve and become more accessible, they hold the promise of revolutionizing disease surveillance, outbreak management, and the development of personalized intervention strategies. Ultimately, the advancements in molecular diagnostics are poised to significantly enhance the health and productivity of cattle populations, contributing to the overall sustainability and welfare of the livestock industry.

References

Smith CJ, Osborn AM. Advantages and limitations of quantitative PCR (Q-PCR)-based approaches in microbial ecology. FEMS Microbiol Ecol. 2009;67(1):6-20.

Reid SM, Ferris NP, Hutchings GH, Zhang Z, Belák S, Alexandersen S. Detection of all seven serotypes of foot-and-mouth disease virus by real-time, fluorogenic reverse transcription polymerase chain reaction assay. J Virol Methods. 2002;105(1):67-80.

Notomi T, Okayama H, Masubuchi H, et al. Loop-mediated isothermal amplification of DNA. Nucleic Acids Res. 2000;28(12):E63.

Liu L, Dong D, Fei D, Liu Y, Cheng C, Zhu Y, et al. Rapid detection of Mycobacterium bovis in cattle and deer by an insulated isothermal PCR assay. Sci Rep. 2018;8(1):13659.

Belák S, Thorén P, LeBlanc N, Vilcek S. Development and evaluation of a real-time Taqman RT-PCR assay for rapid detection of bovine respiratory syncytial virus. J Virol Methods. 2000;90(2):167-177.

Call DR, Davis MA, Sawant AA. Antimicrobial resistance in beef and dairy cattle production. Anim Health Res Rev. 2008;9(2):159-167.

Jiménez DF, Otte J, Pérez ÁM. The social and economic impact of bovine viral diarrhea in the cattle industry of the Americas. Rev Sci Tech. 2019;38(1):193-207.

Schares G, Koethe M, Bangoura B, Geuthner AC, Randau F, Ludewig M, et al. Novel tools for the diagnosis and differentiation of acute and chronic bovine besnoitiosis. Int J Parasitol. 2018;48(5):317-327.

Ruggli N, Tratschin JD, Mittelholzer C, Hofmann MA. Nucleotide sequence of classical swine fever virus strain Alfort/187 and transcription of infectious RNA from stably cloned full-length cDNA. J Virol. 1996;70(5):3478-3487.

Aebischer A, Wernery U, Kinne J, Wernery R, Jose S, Rauschendorfer J, et al. Detection of camelid alphaherpesvirus 2 in dromedary camels with infectious uveitis. Vet Ophthalmol. 2021;24(1):24-31.

Baumann MPO, Flügger M, Sieg M, Pietschmann J, Heusinger A, Reinhardt HC, et al. Development and evaluation of a real-time RT-PCR assay for universal detection of pestiviruses. Vet Microbiol. 2021;254:108998.

Bürki S, Vögtlin A, Fraefel C, Ackermann M. Identification of transcription start sites in the immediate-early ICP22 gene of equine herpesvirus type 1. J Virol. 1997;71(12):9766-9769.

Amanna IJ, Messaoudi I, Slifka MK. Protective immunity following vaccination: How is it defined? Hum Vaccin. 2008;4(4):316-319.

Caswell JL, Bateman KG, Cai HY, Castillo-Alcala F. Mycoplasma bovis in respiratory disease of feedlot cattle. Vet Clin North Am Food Anim Pract. 2010;26(2):365-379.

Kapil S, Yeary TJ. Diagnosis of bovine respiratory syncytial virus infection: An overview. Anim Health Res Rev. 2010;11(2):187-196.

DOI: http://dx.doi.org/10.26549/jaivr.v5i2.15924