An In-Vitro Evaluation of the Efficacy and Functionality of Novel Contact Lens Solution Infused with Guava (Psidium guajava L.) Leaves Extract

Alajar, Alexandra Ysabel D.()
⁠Babani, Jenisha Prakash()
Evangelista, Chylyn Jane F()
Ibasco, Shereen Rose C.()
⁠Uy, Jomar Christian V.()
⁠Gariando, Carol Fe A.()

Abstract

Ensuring an effective antimicrobial activity in contact lens solutions is crucial, as microbial keratitis related to contact lenses can be caused by various pathogens, including Gram-negative and Gram-positive bacteria. Exploring the potential of plant-based antimicrobial agents, such as Psidium guava L. leaves, can lead to innovative solutions in the world of contact lens care. This research focuses on formulating a novel contact lens solution using guava leaf extract, evaluating its in-vitro antimicrobial efficacy, and assessing its impact on contact lenses and lens cases against common bacterial pathogens like Staphylococcus aureus, Pseudomonas aeruginosa, and Serratia marcescens. The study adhered to the standards set by the International Organization for Standardization (ISO) 14729 and 18259. In this quantitative experimental research study, the antimicrobial effectiveness of 45% hydroethanolic guava leaf extract was assessed. The results indicated a significant impact on various microorganisms, as determined by one-way ANOVA analysis. This extract with 0.35% concentration was incorporated as the antimicrobial agent into a novel contact lens solution formulation. The findings of this study demonstrated the remarkable antimicrobial efficacy of the solution against bacterial pathogens, as outlined in ISO 14729 and ISO 18259 for contact lenses and lens cases. These results highlight the good potential of guava extract as a disinfectant agent. By using the antimicrobial properties of guava leaves through the novel contact lens solution, this research has aimed to contribute to the development of safer and more effective plant-based contact lens solutions which can help prevent ocular infections and improve overall eye health.

Keywords

microbial keratitis, antimicrobial efficacy, pathogens, ISO 14729, ISO 18259

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DOI: http://dx.doi.org/10.26549/jams.v8i2.32801