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16s rRNA高通量测序技术在人类医学中的应用进展

溢 杨(康奥科技集团有限公司)
美 黄(康奥科技集团有限公司)
琛 张(康奥科技集团有限公司)
晓彤 刘(康奥科技集团有限公司)
雅南 王(康奥科技集团有限公司)
雪梅 王(康奥科技集团有限公司)
晓光 朱(康奥科技集团有限公司)

摘要

本文主要概述了16s rRNA高通量测序技术的特点和主要操作流程以及Roche 454 GS Life Sciences焦磷酸测序、Illumina Solexa聚合酶测序和ABI SOLiD 连接酶测序3种测序技术的优势、16s rRNA高通量测序技术在口腔、肠道微生物种属鉴定的应用。

关键词

16srRNA;高通量测序;微生物鉴定

全文:

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参考

Delong E.F., G.S. Wickham.N.R. Pace, Phylogenetic stains: ribosomal RNA-based probes for the identification of single cells. Science, 1989. 243(4896): p. 1360-1363.

Woese C.R., O. Kandler.M.L. Wheelis, Towards a natural system of organisms: proposal for the domains Archaea, Bacteria, and Eucarya. Proceedings of the National Academy of Sciences of the United States of America, 1990. 87(12): p. 4576-4579.

姜静, 杨圣辉, 王松灵, 16SrRNA基因及16S-23SrRNA基因间隔区在口腔微生物鉴定中的应用. 北京口腔医学, 2006. 14(4): p. 297-299.

黄菁华, 16S rRNA基因检测技术在肠道微生态研究中的应用. 猪业科学, 2006. 23(8): p. 49-51.

牟丽丽, 涂云华, 明春艳, 等, 几类主要病原需氧放线菌菌属的SecA1基因分析研究. 中国人兽共患病学报, 2016. 32(4): p. 349-355.

Carrasco G., S. Valdezate, N. Garrido, et al., gyrB analysis as a tool for identifying Nocardia species and exploring their phylogeny. Journal of Clinical Microbiology, 2015. 53(3): p. 997-1001.

Lindahl B.D., R.H. Nilsson, L. Tedersoo, et al., Fungal community analysis by high-throughput sequencing of amplified markers – a user's guide. New Phytologist, 2013. 199(1): p. 288-299.

岳桂东, 高强, 罗龙海, 等, 高通量测序技术在动植物研究领域中的应用. 中国科学:生命科学, 2012(2): p. 107-124.

Luo C., D. Tsementzi, N. Kyrpides, et al., Direct comparisons of Illumina vs. Roche 454 sequencing technologies on the same microbial community DNA sample. Plos One, 2012. 7(2): p. e30087.

Huse S.M., Y. Ye, Y. Zhou, et al., A Core Human Microbiome as Viewed through 16S rRNA Sequence Clusters. Plos One, 2012. 7(6): p. e34242.

Ouhara K., H. Komatsuzawa, S. Yamada, et al., Susceptibilities of periodontopathogenic and cariogenic bacteria to antibacterial peptides, β-defensins and LL37, produced by human epithelial cells. J Antimicrob Chemother, 2005. 55(6): p. 888-896.

Noor A.H.N., A. Ahmed, A. Ahmed, et al., Species-level core oral bacteriome identified by 16S rRNA pyrosequencing in a healthy young Arab population. Journal of Oral Microbiology, 2016. 8: p. 31444.

KC Anukam N.A., A Comparative Study of the Oral Micobime Compositions of Healthy Postmenopausal, Premenopausal, and Prepubertal Nigerian Females, Using 16S rRNA Metagenomics Methods. Nigerian Journal of Clinical Practice, 2017. 20(10): p. 1250-1258.

Sonnenburg J.L..F. Bäckhed, Diet–microbiota interactions as moderators of human metabolism. Nature, 2016. 535(7610): p. 56-64.

Round J.L..S.K. Mazmanian, The gut microbiota shapes intestinal immune responses during health and disease. Nature Reviews Immunology, 2009. 9: p. 313.

Leblanc J.G., C. Milani, G.S. de Giori, et al., Bacteria as vitamin suppliers to their host: a gut microbiota perspective. Current Opinion in Biotechnology, 2013. 24(2): p. 160-168.

Stecher B..W.D. Hardt, Mechanisms controlling pathogen colonization of the gut. Current Opinion in Microbiology, 2011. 14(1): p. 82-91.

Almonacid D.E., L. Kraal, F.J. Ossandon, et al., 16S rRNA gene sequencing and healthy reference ranges for 28 clinically relevant microbial taxa from the human gut microbiome. Plos One, 2017. 12(5): p. e0176555.

Alcon-Giner C., S. Caim, S. Mitra, et al., Optimisation of 16S rRNA gut microbiota profiling of extremely low birth weight infants. Bmc Genomics, 2017. 18(1): p. 841.

Deng X., Z. Li, G. Li, et al., Comparison of Microbiota in Patients Treated by Surgery or Chemotherapy by 16S rRNA Sequencing Reveals Potential Biomarkers for Colorectal Cancer Therapy. Frontiers in Microbiology, 2018. 9.

姜洋, 赵秋枫, 王实, 等, 基于16S rRNA序列分析肠道菌群失调与溃疡性结肠炎的相关性. 世界华人消化杂志, 2017(36).



DOI: http://dx.doi.org/10.26549/yzlcyxzz.v1i1.1189

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