电刺激诱导miRNA表达升高促进雪旺细胞的迁移

鸿伟 于(承德医学院，中国)
博 高(承德医学院附属医院，中国)
培 王(承德医学院附属医院手足外科，中国)

摘要

目的：研究电刺激诱导miRNA对雪旺细胞生物学功能的影响。方法：建立大鼠坐骨神经挤压模型，分为电刺激组和不电刺激组，电刺激组每天给予1小时电刺激。于第7天通过实时荧光定量逆转录聚合酶链反应（qRT-PCR）法检测挤压伤处的miR-365-3p、miR-19a-3p、miR-19-3p的表达水平。将RSC96细胞分成模拟物对照、miR-365-3p模拟物、miR-19a-3p和miR-19b-3p模拟物，通过Transwell小室实验检测RSC96细胞迁移能力的变化。并通过TargetScan、miRWalk与miRDB三个数据库预测miR-365-3p、miR-19a-3p、miR-19-3p的靶基因数量。结果：电刺激治疗坐骨神经损伤后，miR-365-3p、miR-19a-3p、miR-19-3p的表达升高。将与miR-365-3p、miR-19a-3p、miR-19-3p及其对照转染进雪旺细胞后，Transwell迁移实验显示，与miR-NC组相比，miR-365-3p组，miR-19a-3p组和miR-19-3p组的促进雪旺细胞的迁移数。TargetScan、miRWalk与miRDB三个数据库预测miR-365-3p、miR-19a-3p、miR-19-3p的靶基因合集数分别为69、78、56。结论：电刺激改变神经损伤修复过程中miR-365-3p、miR-19a-3p、miR-19-3p表达，并且其促进雪旺细胞迁移，实现促进迁移功能可能与数据库预测靶基因相关。

关键词

电刺激；miRNA；雪旺细胞

参考

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DOI: http://dx.doi.org/10.12345/yzlcyxzz.v6i2.12353