丝胶通过上调 Nrf2/HO-1 和 HSP90 的表达保护玻璃化冷冻小鼠卵巢组织

欣 张(承德医学院，中国)
薇菡 舒(承德医学院，中国)
跃兵 乔(承德医学院，中国)

摘要

目的：探究丝胶是否通过调控HSP90和Nrf2/HO-1通路来保护玻璃化冷冻卵巢组织。方法：取30只小鼠卵巢，随机分新鲜组、对照组、0.5%丝胶组、1%丝胶组和0.1mM褪黑素组（M LT），除新鲜组外，其他四组均进行玻璃化冷冻。进行苏木素-伊红染色（HE）及免疫组织化学染色（IHC）。玻璃化冷冻的四组进行蛋白免疫印迹（WB）和实时荧光定量PCR实验。结果：丝胶组可改善卵泡细胞形态，维持较高的形态正常率。IHC显示丝胶组增加Bcl-2的表达，降低Bax和Caspase-3的表达。WB和PCR结果显示丝胶组可有效提高Nrf2/HO-1、HSP90蛋白和mRNA的水平。以上结果1%丝胶效果最佳，与M LT无显著差异。结论：丝胶可提高玻璃化冷冻卵巢的抗氧化能力，降低细胞凋亡，维持卵巢的正常结构，其作用可能与上调Nrf2/HO-1通路及HSP90的表达相关。

关键词

丝胶；卵巢；冷冻保护；HSP90；Nrf2/HO-1

参考

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DOI: http://dx.doi.org/10.12345/yzlcyxzz.v7i6.18532