岩藻多糖对胃腺癌 AGS 细胞 DNA 损伤及细胞周期基因表达的影响

馨予 张(承德医学院，中国)
铮 田(承德医学院，中国)

摘要

目的：探讨岩藻多糖对人胃腺癌AGS细胞DNA损伤应答及细胞周期相关基因mRNA表达水平的影响，为进一步研究其抗胃癌作用机制提供实验依据。方法：体外培养人胃腺癌AGS细胞，取对数生长期AGS细胞进行分组处理，实验分为阴性对照组、岩藻多糖组和阳性对照组。阴性对照组加入等体积完全培养基，岩藻多糖组加入终浓度为500μg/mL的岩藻多糖，阳性对照组加入终浓度为1 μM的5-FU，连续作用72 h。处理结束后提取各组细胞总RNA，经反转录获得cDNA，采用逆转录实时荧光定量PCR检测各组细胞中相关基因mRNA表达水平。结果：与阴性对照组相比，岩藻多糖处理后AGS细胞中CDC25C、CDK1、Cyclin B1等细胞周期相关基因mRNA表达水平降低，p53、p21 mRNA表达水平升高，而AT R、CHK1、PARP1、Histone 3等基因mRNA表达水平变化不明显。结论：岩藻多糖可能通过调控DNA损伤应答及细胞周期相关基因表达，影响AGS细胞周期进程，从而参与抑制胃腺癌细胞增殖。

关键词

岩藻多糖；AGS细胞；RT-qPCR；mRNA表达

参考

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DOI: http://dx.doi.org/10.12345/yzlcyxzz.v9i6.40110