三维培养在研究白血病细胞耐药性方面的应用

健 刘(内蒙古医科大学，中国)
丽梅 李(内蒙古自治区人民医院血液科，中国)
阿荣 金(内蒙古自治区人民医院血液科，中国)

摘要

急性白血病（acute leukemia，AL）是血液系统恶性肿瘤，具有高度的异质性。白血病细胞对化疗药物耐药成为制约白血病治疗的关键难题。体外细胞培养模型是研究急性白血病发生、发展的重要实验方法。与传统的二维细胞培养相比，三维细胞培养模型能更有效地模拟白血病体内微环境，为探索白血病细胞生物学特性提供了良好的实验平台。近年来，越来越多的研究者应用三维细胞培养模型进行细胞耐药性研究，以期提供更有效的潜在治疗药物。论文总结了近年来关于三维培养及其在AL耐药性的体外研究中的应用。

关键词

急性白血病；细胞耐药性；三维培养

参考

Michael J. Brisco, Pamela J. Sykes, Gaynor Dolman, et al. Early Resistance to Therapy during Induction in Childhood Acute Lymphoblastic Leukemia[J].CANCER RESEARCH, 2000(60):521-524.

Amelian A , Wasilewska K , Megias D , et al. Application of standard cell cultures and 3D in vitro tissue models as an effective tool in drug design and development.[J]. Pharmacological Reports, 2017(05):861-870.

Lovitt C J , Shelper T B , Avery V M . Cancer Drug Discovery: Recent Innovative Approaches to Tumor Modeling[J]. Expert Opinion on Drug Discovery, 2016(09):885-894.

Ravi M , Paramesh V , Kaviya S R , et al. 3D Cell Culture Systems: Advantages and Applications[J]. Journal of Cellular Physiology, 2015(01):16-26.

Langhans SA. Three-Dimensional in Vitro Cell Culture Models in Drug Discovery and Drug Repositioning[J]. Front Pharmacol, 2018(09):6.

Knight E, Przyborski S. Advances in 3D cell culture technologies enabling tissue‐like structures to be created in vitro[J]. Journal of Anatomy, 2016(11):746-756.

Knight E,Przyborski S. Advances in 3D cell culture technologies enabling tissue-like structures to be created in vitro[J]. J Anat, 2015(06):746-756.

Mirabelli P,Coppola L,Salvatore M. Cancer Cell Lines Are Useful Model Systems for Medical Research[J]. Cancers(Basel), 2019(08):1098.

Rödling L,Schwedhelm I,Kraus S,et al. 3D models of the hematopoietic stem cell niche under steady-state and active conditions[J]. Sci Rep, 2017(01):4625.

Birgersdotter A,Sandberg R,Ernberg I. Gene expression perturbation in vitro--a growing case for three-dimensional(3D) culture systems[J]. Semin Cancer Biol, 2005(05):405-412.

Laura J. Bray, Marcus Binner, Yvonne Körner, et al. A three-dimensional ex vivo tri-culture model mimics cell-cell interactions between acute myeloid leukemia and the vascular niche[J].Sci Rep, 2017(07):1215-1226.

Guo Jun, Zhao Chunting, Yao Ruyong, et al. 3D culture enhances chemoresistance of ALL Jurkat cell line by increasing DDR1 expression[J]. Exp Ther Med, 2019(03):1593-1600.

DOI: http://dx.doi.org/10.26549/yzlcyxzz.v3i8.6243