结直肠腺瘤相关的长链非编码RNA差异表达谱建立及应用

宝龙 贾(昆明市延安医院病理科，中国)
丽琼 王(昆明市延安医院病理科，中国)
惠荣 朱(昆明市延安医院病理科，中国)

摘要

目的：筛选结直肠腺瘤组织样本中具有差异表达的长链非编码RNA（long non-codingRNA，LncRNA），预测其靶基因，探究靶基因的生物学功能及富集的信号通路。方法：收集临床结直肠手术中腺瘤新鲜样本，高通量测序技术构建LncRNA差异表达谱，生物信息学技术筛选差异表达的LncRNA并进行靶基因预测，GO与KEGG富集分析分别预测靶基因的生物学功能及富集的信号通路。结果：与正常组织样本相比，结直肠腺瘤组织样本中筛选出219个显著差异表达LncRNA（119个上调，99个下调)。功能性分析表明：与结直肠腺瘤相关的GO条目1242条；KEGG富集的信号通路32条。结论：结直肠腺瘤样本中差异表达的LncRNA可能通过靶向调控其靶基因参与调控多种生物学功能及结直肠腺瘤相关的信号通路，在结直肠腺瘤形成过程中可能发挥重要作用。

关键词

结直肠腺瘤；长链非编码RNA；基因

参考

Bray F, Ferlay J, Soerjomataram I, et al. Global cancer statistics 2018: OBOCAN stimates of incidence and mortality worldwide for 36 cancers in 185 countries[J].CA Cancer J Clin,2018(68): 4-424.

Chen W, Zheng R, Baade P D, et al. Cancer statistics in China[J]. Ca A Cancer Journal for Clinicians, 2016, 66(2):115-132.

Bjerrum A, Lindebjerg J, Andersen O, et al. Long-term risk of colorectal cancer after creen-detected adenoma: experiences from a Danish gFOBT-positive screening cohort[J].Cancer, 2020(147): 940-947.

Citarda F, Tomaselli G, Capocaccia R, et al. Efficacy in standard clinical practice of colonoscopic polypectomy in reducing colorectal cancer incidence[J]. Gut,2001(48):812-815.

Kelloff GJ, Schilsky RL, Alberts DS, et al. Colorectal adenomas: a prototype for the use of surrogate end points in the development of cancer prevention drugs[J].Clin Cancer Res,2004(10):3908-3918.

Mercer TR, Dinger ME, Mattick JS. Long non-coding RNAs: insights into functions[J]. Nature Reviews Genetics, 2009,10(3):155-159.

Clark MB, Mattick JS. Long noncoding RNAs in cell biology[J]. Seminars in Cell & Developmental Biology, 2011,22(4):366-376.

Wilusz JE, Sunwoo H, Spector DL. Long noncoding RNAs: functional surprises from the RNA world[J]. Genes & Development, 2009,23(13):1494-1504.

Thomson DW, Dinger M E. Endogenous microRNA sponges: evidence and controversy[J]. Nature Reviews Genetics, 2016,17(5):272-287.

杨倩,黄宏斌,龚朝建,等.lncRNAs参与基因表达调控机制的研究进展[J].中南大学学报(医学版),2014,39(1):91-95.

Fearon ER, Vogelstein B. A genetic model for colorectal tumorigenesis.[J].Cell,1990,61(5):759-767.

Khalil, M A , Azhar, et al. Aetiology, maternal and foetal outcome in 60 cases of obstetrical acute renal failure[J].Ayub Medical College, Abbottabad,2009(21):46-49.

Wilusz J E, Sunwoo H, Spector, et al.Long noncoding RNAs: functional surprises from the RNA world[J].Genes Dev,2009(23):1494-1504.

Kurokawa R, Rosenfeld MG, Glass CK. Transcriptional regulation through noncoding RNAs and epigenetic modifications[J]. Rna Biology, 2009,6(3):233-236.

Yin Q, Wu A, Liu M . Plasma Long Non-Coding RNA (lncRNA) GAS5 is a New Biomarker for Coronary Artery Disease[J]. Medical Science Monitor International Medical Journal of Experimental & Clinical Research, 2017(23):6042-6048.

Kapranov P, Cheng J, Dike S, et al. RNA Maps Reveal New RNA Classes and a Possible Function for Pervasive Transcription[J]. Science, 2007,316(5830):1484-1488.

Hirata H, Hinoda Y, Shahryari V, et al. Long Noncoding RNA MALAT1 Promotes Aggressive Renal Cell Carcinoma through Ezh2 and Interacts with miR-205[J]. Cancer Research, 2015, 75(7):1223-1225.

Koyo N, Reo M, Takeshi N, et al. Screening for long noncoding RNAs associated with oral squamous cell carcinoma reveals the potentially oncogenic actions of DLEU1[J]. Cell Death & Disease, 2018, 9(8):826-828.

Christiana A , Uzozie, Nathalie, et al. Targeted Proteomics for Multiplexed Verification of Markers of Colorectal Tumorigenesis.[J]. Molecular & cellular proteomics : MCP, 2017(3):3112-3117.

Nissan A, Stojadinovic A, Mitrani-Rosenbaum S, et al. Colon cancer associated transcript-1: a novel RNA expressed in malignant and pre-malignant human tissues[J].Cancer,2012,130(7):1598-1606.

Sonja I, Berndt John D, Potter Aditi Hazra Meredith Yeager Gilles Thomas Karen W, et al. Pooled analysis of genetic variation at chromosome 8q24 and colorectal neoplasia risk[J]. Human Molecular Genetics, 2008,17(17):2665.

Li, Zuguo. MALAT-1: a long non-coding RNA and its important 3’ end functional motif in colorectal cancer metastasis.[J]. International Journal of Oncology, 2011(6):132-137.

Graham LD, Pedersen SK, Brown GS, et al. Colorectal Neoplasia Differentially Expressed (CRNDE), a Novel Gene with Elevated Expression in Colorectal Adenomas and Adenocarcinomas[J]. Genes, Cancer, 2011,2(8):434-436.

DOI: http://dx.doi.org/10.12345/yzlcyxzz.v4i12.9759